DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF MOSAPRIDE IN BULK AND PHARMACUTICAL FORMULATION

To develop simple, economical, precise UV methods for the estimation of Mosapride in bulk and pharmaceutical formulations. The methods are based on zero and first order spectroscopic techniques. Mosapride shows the maximum absorbance at 264nm (Method A), and in first order derivative spectra showed zero crossing point at 264nm with a sharp peak at 254nm when Δג =10 (Method B). Drug followed the linearity in the range of 5-30µg/ml for both methods with correlation coefficient (r2) of 0.999. The results of analysis have been validated statistically and recovery studies confirmed the accuracy of the proposed method. The method was validated as per the International Conference on Harmonization (ICH) guidelines. The proposed method is recommended for routine analysis since it is rapid, simple, accurate and sensitive. Keywords: Mosapride citrate, UV spectrophotometry, Derivative spectra