Rapid identification of viruses is needed to monitor the blood supply for emerging threats. Here we present a method that meets these criteria and allows for the shotgun sequencing of novel, uncultured DNA viruses directly from human blood. This method employs selection based on the physical properties of viruses combined with sequence-independent amplification and cloning. We show that both single- and double-stranded DNA viruses can be recovered from blood samples using this approach. In addition, we report the discovery of novel anellovirus sequences in the blood of healthy donors. PCR primers designed to amplify these novel anellovirus sequences were then used to verify the presence of these viruses in the general donor population
<p><b>A–B</b>. Patient plasma (<b>A</b>) and NP (<b>B</b>) specimens were assayed for TTV, TTMDV, an...
Viral infections cause many different diseases stemming both from well-characterized viral pathogens...
We have developed a full genome virus detection process that combines sensitive nucleic acid prepara...
Rapid identification of viruses is needed to monitor the blood supply for emerging threats. Here we ...
<div><p>The characterization of the blood virome is important for the safety of blood-derived transf...
Because of the constant threat posed by emerging infectious diseases and the limitations of existing...
Because of the constant threat posed by emerging infectious diseases and the limitations of existing...
Human blood metagenomics has revealed the presence of different types of viruses in apparently healt...
DNA microarray-based approaches to viral detection and discovery help overcome many limitations of t...
Background. Characterisation of human-associated viral communities is essential for epidemiological ...
Abstract Background Sequence-specific PCR is the most common approach for virus identification in di...
The use of high-throughput sequencing (HTS) to identify viruses in biologicals differs from current ...
Although nearly one fifth of all human cancers have an infectious aetiology, the causes for the majo...
High-throughput sequencing furnishes a large number of short sequence reads from uncloned DNA and ha...
Unveiling origin of infectious diseases with unknown etiology is one of the major issues in contempo...
<p><b>A–B</b>. Patient plasma (<b>A</b>) and NP (<b>B</b>) specimens were assayed for TTV, TTMDV, an...
Viral infections cause many different diseases stemming both from well-characterized viral pathogens...
We have developed a full genome virus detection process that combines sensitive nucleic acid prepara...
Rapid identification of viruses is needed to monitor the blood supply for emerging threats. Here we ...
<div><p>The characterization of the blood virome is important for the safety of blood-derived transf...
Because of the constant threat posed by emerging infectious diseases and the limitations of existing...
Because of the constant threat posed by emerging infectious diseases and the limitations of existing...
Human blood metagenomics has revealed the presence of different types of viruses in apparently healt...
DNA microarray-based approaches to viral detection and discovery help overcome many limitations of t...
Background. Characterisation of human-associated viral communities is essential for epidemiological ...
Abstract Background Sequence-specific PCR is the most common approach for virus identification in di...
The use of high-throughput sequencing (HTS) to identify viruses in biologicals differs from current ...
Although nearly one fifth of all human cancers have an infectious aetiology, the causes for the majo...
High-throughput sequencing furnishes a large number of short sequence reads from uncloned DNA and ha...
Unveiling origin of infectious diseases with unknown etiology is one of the major issues in contempo...
<p><b>A–B</b>. Patient plasma (<b>A</b>) and NP (<b>B</b>) specimens were assayed for TTV, TTMDV, an...
Viral infections cause many different diseases stemming both from well-characterized viral pathogens...
We have developed a full genome virus detection process that combines sensitive nucleic acid prepara...