Structural basis of the stereoselective formation of the spirooxindole ring in the biosynthesis of citrinadins
- Zhiwen Liu
- Fanglong Zhao
- Boyang Zhao
- Jie Yang
- Joseph Ferrara
- Banumathi Sankaran
- B. V. Venkataram Prasad
- Biki Bapi Kundu
- George N. Phillips
- Yang Gao
- Liya Hu
- Tong Zhu
- Xue Gao
Publication date
July 2021
DOI Publisher
Springer Science and Business Media LLC Journal
Nature CommunicationsAbstract
Prenylated indole alkaloids contain spirooxindole rings with a 3R or 3S carbon stereocenter, which determines their bioactivities, but the biocatalytic mechanism controlling the 3R- or 3S-spirooxindole formation was unclear. Here, the authors report the biochemical and structural characterization of the oxygenase/semipinacolase CtdE that catalyses the 3S-spirooxindole construction in the biosynthesis of 21R-citrinadin A
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