Additional file 1 of Sodium valproate increases activity of the sirtuin pathway resulting in beneficial effects for spinocerebellar ataxia-3 in vivo

Additional file 1: Higher doses of sodium valproate (valproate) affect morphology of transgenic MJD zebrafish. A Brightfield images of 6 day old i) non-transgenic, ii) EGFP-Ataxin-3 23Q, iii) EGFP-Ataxin-3 84Q vehicle, iv) 3.125 µM valproate treated, v) normal 6.25 µM valproate EGFP-Ataxin-3 84Q and vi) abnormal 6.25 µM valproate treated EGFP-Ataxin-3 84Q zebrafish larvae. Scale bar represents 500 µm. B Percentage of normal morphology reveals 6.25 µM valproate treated EGFP-Ataxin-3 84Q larvae had decreased normal morphology (****p < 0.0001; n = 6). C Non-transgenic zebrafish treated with 3.125 µM valproate between 1 and 6 days of age resulted in no changes to the distance swum compared to the vehicle treated control (p = 0.546; n = 68–70). Data represents mean ± SEM. Comparisons of the percentage of normal morphology were analysed using a one-way ANOVA followed by a Tukey post-hoc analysis and swimming distance was analysed using an unpaired t-test