Additional file 5 of Sodium valproate increases activity of the sirtuin pathway resulting in beneficial effects for spinocerebellar ataxia-3 in vivo

Additional file 5: Cells expressing polyglutamine expanded human ataxin-3 have decreased levels of SIRT1 protein. A Immunoblot of HEK293 cells stably expressing an empty vector, ataxin-3 28Q and ataxin-3 84Q probed for human ataxin-3 and SIRT1. All of the cells, including those expressing just the empty vector, carried the endogenous human ataxin-3 band, which ran at a similar height to the human ataxin-3 28Q band. Cells expressing human ataxin-3 84Q showed decreased SIRT1 levels. B Densitometric analysis of SIRT1 levels confirmed decreased levels of SIRT1 in the polyQ expanded ataxin-3 cells compared to the ataxin-3 23Q cells (p = 0.0213, n = 8–9 independent experiments). Data represents mean ± SEM. Statistical analysis performed was a one-way ANOVA followed by a Tukey post-hoc analysis