DAZ-1, GLD-1, OMA-1/2, and LIN-41 regulate spatiotemporal expression pattern of MEX-3.

(A) DIC and fluorescence images of wild type GFP::MEX-3 animals from the control RNAi. (B) DIC and fluorescence images of GFP::MEX-3 animals after daz-1 knockdown. GFP::MEX-3 was significantly increased in the mitotic distal end. (C) DIC and fluorescence images of GFP::MEX-3 animals after gld-1 knockdown. GFP::MEX-3 expression was derepressed in the meiotic region. (D) DIC and fluorescence images of GFP::MEX-3 animals after lin-41 knockdown. GFP::MEX-3 was de-repressed in the loop region. (E) DIC and fluorescence images of GFP::MEX-3 animals after oma-1/2 knockdown. GFP::MEX-3 was significantly increased in the oocytes. (F) quantitative analysis of fluorescence intensity after daz-1 knockdown (n = 9/15). For all the images from the RNAi, a line with a width of 30 pixels was drawn along the entire germline and fluorescence intensities were binned (20 bins). Data are shown as the mean fluorescence intensity ± standard deviation (SD). A two tailed student t-test was performed to compare the means for each bin from control animals and the RNAi condition to assess significance. For RNAi conditions that have the same control, a one-way unstacked ANOVA was used to assess the overall significance, then Bonferroni adjusted p-values were calculated by multiplying pairwise Fisher´s LSD test p-values by the number of hypotheses tested. All p-values for this figure are reported in S4 Table. (G) quantitative analysis of fluorescence intensity after gld-1 knockdown (n = 7/13). (H) quantitative analysis of fluorescence intensity after lin-41 knockdown (n = 17/17). (I) quantitative analysis of fluorescence intensity after oma-1/2 knockdown (n = 9/9). (J) quantitative analysis of nuclear GFP::MEX-3 of the oma-1/2 RNAi animals. Nuclear fluorescence intensity was divided by the cytoplasmic fluorescence intensity for each oocyte. Each dot represents the averaged ratios from the two most proximal oocytes in an individual animal. (*) indicates statistical significance, adjusted p-value ≤ 0.05. (****) indicates statistical significance, p-value ≤ 0.0005. Scale bar = 30 μm. (TIF)