Influence of Surface Chemistry on Microtextured Titanium on the Osteoblast Cell Response

Jung Hwa Park presented a lecture at the Nano@Tech Meeting on December 6, 2011 at 12 noon in room 1116 of the Marcus Nanotechnology Building.Jung Hwa Park is a 4th year graduate student in Materials Science and Engineering.Runtime: 20:44 minutesThe aim of this study was to examine the possibility to increased surface wettability of microstructured Ti surfaces through polyelectrolyte modifications without changing surface microstructure and whether these chemical modifications alter osteoblast maturation. Polyelectrolytes [chitosan (CHI), poly(L-glutamic acid) (PGA), and poly(L-lysine) (PLL)] were used to coat Ti surfaces with different surface roughness (PT, Ra ≤ 0.4 μm and SLA, Ra > 3.5 μm). Surfaces were characterized using X-ray photoelectron spectroscopy, scanning electron microscopy, profilometry, and contact angle. MG63 osteoblast-like cells were cultured on uncoated or the chemically-modified surfaces. At confluence, cells number, alkaline phosphatase specific activity, and osteocalcin levels were analyzed. The polyelectrolyte modifications increased surface wettability without changing surface microstructure. This change had effect on osteoblast differentiation which was material dependent