Investigation of The Interaction Between NS1 and SP1 and its Role in Transcriptional Transactivation of Human Parvovirus B19

The research on parvovirus B19 (B19V) has been a popular area since it was known in 1975. It causes several clinical illnesses such as the Fifth disease, erythema infectiosum and autoimmune hepatitis. However, less attention has been given to the non-structural protein (NS1) of B19V, which is a main replicative protein encoded by the virus genome. In this thesis, background information about B19V NS1 is studied along with the potential scientific approaches to reveal the molecular mechanism of transcription transactivation by NS1. Purification of the C-terminal domain (CTD) of NS1 in two tagged constructs, hMBP-CTD and GST-CTD, has been conducted. Finally, the preferable purification methods have been discussed