Evaluation of the composition of phenolic compounds in Artemisia abrotanum L. during different vegetation stages

In order to solve the problem of increasing the diversity of medicinal plants and biologically active compounds and their application in medicine, pharmacy, is being studied Artemisia L. genus [1]. Previous studies have shown that the Artemisia abrotanum L. accumulate high amounts of phenolic compounds (anthocyanins, phenolic acids, flavan-3-ols, flavonols), organic acids and mineral substances [2]. A. abrotanum essential oil have anti-inflammatory, expectorant, spasmolytic, antiseptic and antimicrobial effects [3]. The object of investigation was A. abrotanum a perennial medicinal, aromatic semi-shrub of Asteraceae (Bercht. & J. Presl) family. The aim of this study was to determine the composition and content of phenolic compounds in ethanolic extracts of Artemisia abrotanum L. raw material during different vegetation stages. The raw material of Artemisia abrotanum L. were collected during different vegetation stages in the Spice-Milliferous Plants Collection of Medicinal Plants of the Scientific Sector of Medicinal and Aromatic Plants, Scientific Department of Botanical Garden at Vytautas Magnus University in 2019. The samples were extracted with 70 % (v/v) ethanol in an ultrasonic bath for 30 minutes. The analysis of phenolic compounds was performed on the basis of high-performance liquid chromatography method. During the study, the following glycosides of the flavonol group were identified: luteolin-7-rutinoside, luteolin-7-glicoside, luteolin-3-rutinoside, isorhamnetin-3-rutinoside, rutin. In addition, compounds of phenolic acids group were identified, such as chlorogenic acid, caffeic acid, neochlorogenic acid, 4-O-caffeoylquinic acid, 3,4-di-caffeoylquinic acid, 3,5-di-caffeoylquinic acid and 4,5-di-caffeoylquinic acid. Of all identified compounds chlorogenic acid predominated during all vegetation stages. In conclusion, the results of this study will provide new data about the composition and content [...]Lietuvos sveikatos mokslų universitetas. Medicinos akademijaVytauto Didžiojo universiteta