Study of recombinant protein production from transgenic Nicotiana tabacum plant cells in a perfusion bioreactor

Production of recombinant proteins in cell cultures -- Bacterial cells -- Mammalian cells -- Plant cells -- Advantages in plant cell cultures -- Plant cell culture characteristics -- Recombinant protein production and promoters -- Improving recombinant protein production -- Protein recovery ans downstream processing -- Continuous production of murine IgG1 by Nicotiana tabacum suspension cells in a recirculation loop bioreactor -- Suspension cell culture -- Bioreactor configuration -- Medium recirculation and perfusion strategies -- Analytical -- Ions -- Glucose -- Soluble proteins -- IgG1 antibody -- Cell growth -- The recombinant mouse antibody IgG1 can be continuously extracted -- Nutrient concentration changes