PC1 is required for IGF-1-induced cardiomyocyte hypertrophy.

Cardiomyocytes were transfected with scrambled (Scr) or PC1 siRNA (siPC1), incubated for 24 h, and then treated with IGF-1 (10 nM) or vehicle for 24 h. (A) Representative Western blot of polycystin-1 (PC1) and GAPDH as a loading control. The lower panel shows the PC1/GAPDH ratio. (B) Bar graph of polycystic kidney disease 1 (Pkd1) mRNA levels. (C) Upper panel shows representative Western blots of β-MHC and GAPDH. The graph in the lower panel shows the β-MHC/GAPDH ratio calculated by densitometric analysis. (D) Brain natriuretic peptide (BNP) mRNA levels determined by qRT-PCR are expressed relative to Pabpn1 mRNA. (E) [3H]-leucine incorporation in cardiomyocytes. (F) Bar graph shows Pkd1 mRNA levels after IGF-1 stimulus. Values are shown as mean ± SD (n = 3-6). *p&p<0.05 vs. Scr+IGF-1.