IGF-1-induced hypertrophic changes in cardiomyocyte morphology are mediated by PC1.

Cardiomyocytes were transfected with scrambled (Scr) or PC1 siRNA (siPC1), incubated for 24 h, and then treated with IGF-1 (10 nM) or vehicle for 24 h. (A) Representative images of cardiomyocytes stimulated with IGF-1 and stained with rhodamine-phalloidin. (B) Fluorescence densitometric profiles of the lines depicted in (A), showing sarcomere density in IGF-1-stimulated cardiomyocytes pretreated with Scr or siPC1. Bar graphs show cardiomyocyte (C) cell area, (D) cell perimeter and (E) sarcomerization. Values are shown as mean ± SD (n = 3–6). *p&p<0.05 vs. Scr+IGF-1.