CD40L expression by CD4(+) but not CD8(+) T cells regulates antiviral immune responses in acute LCMV infection in mice

CD40-CD40 ligand (CD40L) signaling plays multiple indispensable roles in cellular and humoral immunity. Impaired memory T-cell responses in the absence of CD40L have been well documented, but the requirement of this interaction for efficient priming of CD8(+) T cells especially under inflammatory conditions has been under debate. In contrast to previous publications, we report here that virus-specific CD8(+) T-cell responses as well as viral clearance are affected not only in the memory but also in the effector phase in CD40L(-/-) mice infected with lymphocytic choriomeningitis virus (LCMV) Armstrong strain. Interestingly, a considerable part of the LCMV-specific effector andmemory T cells consists of CD40L(+) CD8(+) T cells. However, deficiency of CD40L in CD8(+) T cells did influence neither the quantity nor the quality of primary T-cell responses in LCMV infection. Virus-specific CD8(+) T cells in conditional knockout mice, with a selective deletion of the CD40L in CD8(+) T cells, were fully functional regarding cytokine production and efficient pathogen clearance. Thus, our results unambiguously demonstrate that while CD40L is critical to generate effective primary CD8(+) T-cell responses also under inflammatory conditions, CD40L expression by CD8(+) T cells themselves is dispensable in acute LCMV infectio