Whole slide inclusion analysis defines the exponential nature of AAT accumulation as globules and its relationship with fibrosis.

(A). Stepwise representation of quantitative analysis of PAS-D inclusions 200 um scale bar (B) Selection of algorithm, points represent % area of a single biopsy occupied by inclusions measured by computer (C) or human observers (1, 2) bars represent median and IQR points represent % area measured from whole slide images. (C) Comparison between pathologist-based qualitative staging and quantitative analysis as median PAS-D % area and IQR (D) PAS-D % area compared to fibrosis stages bars represent median and IQR statistical testing via Kruskal-Wallis test with Dunn’s multiple comparisons (* pE) Separation of population based on quartiles and outliers (outliers defined as inclusion % area greater than or equal to Q3+1.5*IQR).