Type IV pilus-dependent phenotypes are maintained in <i>X</i>. <i>citri</i> strains carrying fluorescent chimeras.
- Edgar E. Llontop (7252868)
- William Cenens (282027)
- Denize C. Favaro (1975516)
- Germán G. Sgro (6716702)
- Roberto K. Salinas (221476)
- Cristiane R. Guzzo (11278921)
- Chuck S. Farah (6716723)
Publication date
August 2021
DOI Publisher
Public Library of Science (PLoS)Abstract
A) Time-lapse of in X. citri msfGFP-FimX, msfGFP-PilZ, msfGFP-PilB and PilQ-msfGFP strains exhibiting twitching motility. The time lapse interval (h) is indicated for each frame. Images were taken by using a phase-contrast microscope. Scale bar, 20 μm. Also see S1–S4 Movies. B) Phage ΦXacm4-11 infection assays for X. citri msfGFP-FimX, msfGFP-PilZ, msfGFP-PilB, PilQ-msfGFP, mCherry-FimX/msfGFP-PilZ, mCherry-FimX/PilQ-msfGFP and mCherry-FimX/msfGFP-PilB strains. Dark plaques are indicative of phage-induced bacterial lysis in a confluent culture background. (TIF)</p
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