LdAg-based prophylactic vaccine decreases <i>Leishmania</i> parasitic burden.

(A) Scheme representing the prophylactic vaccine protocol against VL. All mice groups were infected; PBS group represents non-vaccinated mice, ID LdAg group represents intradermally vaccinated mice and IN LdAg represents intranasally vaccinated mice. Mouse image is from smart.servier.com. (B, C) Spleen (B) and liver (C) weights measured immediately after dissection of different mice groups. (D-F) The effect of ID and IN LdAg vaccine on L. donovani parasite count in livers (D), spleens (E) and bone marrows (F) estimated by qPCR in 75 ng of tissue gDNA. Data are representative of two independent experiments and are presented as means ± SEM (n = 5–7; one-way ANOVA).