PHD domains in PfPHD1 and PfPHD2 proteins.

(A) Sequence of four PHD domains in PfPHD1, C and H amino acid residues in the PHD domain are highlighted underneath the sequence. PHD-SF: PHD superfamily; ePHD: elongated PHD domain, PHD_TAF3: TAF3 type PHD domain. (B) Sequences of four PHD domains in PfPHD2. (C) Alignment of PfPHD1 PHD_TAF3 domain with other known authentic PHD domains which bind H3K4me3/2. The alignment shows the conserved Zinc-binding residues in light gray for Zinc 1 and dark gray for Zinc 2, and the two core β-strands in green. The residues involved in H3K4me3 recognition are labeled I through V (forming the aromatic cages) and the aromatic residues in the recognition cage are shadowed in green. MLL1: mixed-lineage leukemia-1; JARID1A: jumonji, AT-rich interactive domain 1A; PYGO: pygopus homolog 1; BPTF: bromodomain PHD finger transcription factor; PHF2: PHD finger protein 2; Yng1: yeast homolog of mammalian ING1; ING4: inhibitor of growth protein 4; TAF3: transcription initiation factor TFIID subunit 3. (PDF)