Variable tomographic scanning with wavelength scanning digital interface holography

A series of holograms is recorded by synchronizing a camera with laser pulses under the control of a digital delay generator. Amplitude and phase images are calculated while image distances are adjusted for the best focus on the object under observation. The amplitude and phase images are reconstructed while adjusting the image distances over a predetermined range to maintain the object in focus. Numerical superposition of a plurality of holographic fields taken with varying wavelengths provides high resolution microscopic three-dimensional imaging. Numerical reconstruction is based on an angular spectrum method that enables calculation of the image at any distance from the hologram plane. Wavelength scanning digital interference holography also enables image reconstruction along an arbitrarily tilted plane