Video 2 from Go with the FLOW: visualizing spatiotemporal dynamics in optical widefield calcium imaging

An example pan cortical wave (left pane) in widefield imaging data recorded from a developing mouse and the corresponding FLOW portrait (right pane). This wave event is depicted in Figure 6B as wave ii. The video is played at 20 frames per second such that one second of video corresponds to one second of recording. The FLOW portrait was computed using an integration length of 2 seconds (40 frames), and a visualization threshold of 0.92