Effects of alcohol intoxication for 1 hour on skeletal muscle fatigue development in male and female mice.

Force decline from initial stimulation (%) and absolute force production (mN) during the fatigue protocol at 1 hour for Control (CON) females (n = 5) and Alcohol (EtOH) females (n = 5) (A and C), as well as Control (CON) males (n = 5) and Alcohol (EtOH) males (n = 6) (B and D). The rate of force development and ½ relaxation times at various stimulations (1, 15, 30, 60) during the fatigue protocol are also shown for females (E, G) and males (F, H). Force recovery following a 5-minute rest period shown as a percentage of initial force are shown for females (I, K) and males (J, L). Data were analyzed using two-way ANOVA or unpaired t-tests and are presented as mean ± SE for Figures A-D, and as mean ± SD for Figures E-L. In panels A, B, C, D, p≤ 0.0001 for the main effect of treatment or force, with all also having a significant interaction (A and B, pp<0.05).