Expression of fibroblast growth factor 10 (FGF10) in Graves′ orbitopathy (GO) and in non-GO tissues and cells.

(A) Expression of fibroblast growth factor 10 (FGF10) and FGF receptor 2b (FGFR2b) mRNA in GO and non-GO orbital tissues. (B) Microscopy images showing the expression of FGF10 (green) and live-cell nuclei (4’,6-diamidino-2-phenylindole dihydrochloride; blue) in GO and non-GO orbital tissues. (C) Expression of FGF10 protein in GO and non-GO orbital fibroblasts (OFs). Bar graphs show the relative density of FGF10 normalized to the level of β-actin and are represented as means ± standard deviation. (D) Gene expression of fibronectin, collagen Iα, and α- smooth muscle actin in GO and non-GO OFs. Experiments were performed at least three times using different strains. *p < 0.05, **p < 0.01.