Reproducibility of MOA-seq coverage and footprints from bio-replicate analysis.

Comparison of the two bio-replicates, rep1 and rep2, each from ABCD combined (Methods). (A) MOA-seq read coverage (Mc, light orange), MOA footprint coverage (Mfc, dark orange), MNase control coverage (CTRL-c), and MNAse control footprint coverage (CTRL-fc) are shown for two representative areas around the sdg118 gene (left, Zm00001eb363830) and a DUF-like gene (right, Zm00001eb364010). For each example, the peaks for the combined datasets (Mp and Mfp) are shown. (B) Venn diagram of rep1 and rep2 shows the 1-to-1 quantification of overlapping bases within overlapping peaks. The total shared base pairs (7.42 Mbp) represent 69.7% of Rep1 and 67.8% of Rep2 bases in their respective peaks. (C) Biological replicate correlation analysis for replicate 1 (rep1), replicate 2 (rep2) and the MNase control (CTRL).