Analysis of real cell data with varying degree of bifurcating protrusions indicates polar and multiple edge representation in PC2.

(A, B) Mapping of cell shape with anterior-posterior elongation without lateral pseudopods (Dictyostelium prespore cell-type). Cell-to-cell variation (A) and a representative temporal variation of a single cell (B). Blue circled regions represent 95% confidence eclipses for the mean of all combined timeseries (dotted) and the mean of individual cells (filled). (C, D) Mapping of mouse T cells in the PC1-PC2 space. Filled rhombuses: T helper 1 (C) and regulatory T cell (D). Circled regions in the background indicate the reference data in Fig 1B (aggregation-stage Dictyostelium dark green (dark green), HL60 (dark red) and fish keratocyte (yellow)). (TIF)