Monocyte cell type-specific differentially methylated loci associated with acute HIV infection.

(a) Diagram of experimental design. Created with BioRender.com (b) Manhattan plot of differentially methylated loci associated with AHI in monocytes displayed across chromosomes. P values transformed using -log10(P) (c) Volcano plot displaying HUGO gene symbols and related CpG ID (cg#) for top hypomethylated and hypermethylated sites. Difference in DNA methylation displayed as delta beta values plotted against P values transformed using -log10(P). NS: non-significant. (d-q) Plots demonstrating changes in DNA methylation in AHI Fiebig I (red), Fiebig II (blue) Fiebig III-V (purple) compared to HIV- (green) at single CpGs sites related to specific genes or intergenic regions of the genome displayed in bold italic above CpG ID (cg#). Significance determined comparing HIV- vs. AHI and using FDR adjusted P-values <0.05.