Male and female coverage in the part of the <i>M</i>. <i>picta</i> LG12 corresponding to the region shown in S7 Fig), and coverage values relative to the values for an autosome, LG15.

The red horizontal lines indicate ratios of 1, 0.5 and 0. The values were estimated from genome sequence data on 166 and 157 females, from samples from 11 natural populations (S1 Table). The relative coverage values were estimated for each sex separately for each population, as different populations might yield different results in the sequencing, and the overall means for the two sexes were then calculated. In the region between 20 and 25 Mb the sex difference in coverage (M/F coverage ratio) is similar to the value of 0.5 for the rest of LG12 (see Fig 2 and S7 Fig), but the relative (M/A and F/A) values are lower in both sexes). In the most distal region, where M/F coverage values are variable, and often high in one sex or the other. 40 of the 64 genes have lower relative coverage in males than females, ten higher in males, and 14 have similar coverage in both sexes. Note that our analyses excluded low coverage and low quality sequences (as described in S1 Methods 1). (TIFF)