MTs orchestrate HBV virion secretion by regulating MVBs morphogenesis.
- Lei Yue (276252)
- Chang Li (133800)
- Mingzhu Xu (4239415)
- Min Wu (41570)
- Jiahui Ding (5218217)
- Jiangxia Liu (11250890)
- Xiaonan Zhang (538829)
- Zhenghong Yuan (12153)
Publication date
August 2021
DOI Publisher
Public Library of Science (PLoS)Abstract
(A) HepAD38 cells were fixed and processed for detecting HBV (-) DNA, HBsAg and MVBs specific marker CD63. Specific enlargement of the area within the white-outlined box, colocalization of (-) DNA, HBsAg and CD63 were presented by white arrows. Scale bar, 4 μm. (B) Quantification of images in DMSO-treated cells and analyzing the relationship between the mean volume of CD63+ vesicles and (-) DNA puncta in each cell. (C, D) Mean volume and puncta of CD63+ vesicles in a single cell level were measured by Huygens. Analyzing (-) DNA and CD63+ positive (E), HBsAg and CD63+ positive (F) colocalization percent per cell within 3 μm. More than 15 cells per group was counted. ****P < 0.0001 (Mann-Whitney U-test).</p
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