IL-10 Receptor Neutralization-Induced Colitis in Mice: A Comprehensive Guide

In the healthy gut, interleukin-10 receptor (IL-10R) signaling maintains immunologic tolerance to microbial antigens. In this protocol, we outline the neutralization of the IL-10R as a novel mouse model of inflammatory bowel disease. With this method, colitis develops in a progressive fashion by 4 weeks and exhibits the typical features of IBD: colomegaly, splenomegaly, heightened intestinal and systemic inflammation, and histological visualization of goblet cell loss, epithelial hyperplasia, distorted crypt structure, and mucosal thickening