Fig 2 -

Clustal omega alignment of (A) Human and P. furiosus PCNAs (B) P. furiosus PCNA and K. gabonensis beta clamp (C) E. coli beta clamp and K. gabonensis beta clamp (D) E coli beta clamp and human PCNA. When PCNA and beta clamps were aligned, domain 1 of PCNA was aligned to domain 2 of beta clamp and domain 2 of PCNA was aligned to domain 3 of beta clamp. Since (A) and (C) align PCNA to PCNA and beta clamp to beta clamp (domains 2 and 3 aligned), they show the highest degree of similarity and identity. (B) P. furiosus PCNA and K. gabonensis beta clamp also display a high degree of sequence identity and sequence similarity that is statistically significant. In the figure * represents sequence identity,: represents high sequence similarity (similar category of amino acids) and. represents low sequence similarity. (A) and (D): Color represents the secondary structures present in beta clamps of E. coli and PCNA of Human and P. furiosus. Yellow represents beta sheets, green represents helix and red represents turns.