Performance evaluation of ion exchange and affinity chromatography for HbA(1c) estimation in diabetic patients with HbD: A study of 129 samples

Objectives: To compare ion exchange and boronate affinity chromatography for HbA(1c) estimation in Patients with type I and II diabetes having hemoglobin D. Design And Methods: Systems based on ion exchange and boronate affinity chromatography were evaluated and compared for their performance forHbA(1c) estimation in Patients with homozygous and heterozygous D disease. Results: Boronate affinity chromatography shows least interference by HbD in heterozygous as well as homozygous diabetic Patients for HbA(1c) estimation. Conclusion: The use of boronate affinity chromatography was found to be helpful in evaluating glycemic control in diabetic subjects with HbD