An amperometric cholesterol biosensor based on immobilization of cholesterol oxidase onto titanium dioxide nanoparticles

Cholesterol is an important amphipathic molecule and essential structural constituent of all animal cells. It is made up of a hydrocarbon chain, four hydrocarbon ring structure and a hydroxyl group. It is also used in Vitamin D biosynthesis. Cholesterol is of two types: LDL (low-density lipoproteins, bad cholesterol) and HDL (high-density lipoproteins, good cholesterol. High cholesterol increase risk of coronary heart disease. Proposed work describes a unique approach of immobilizing cholesterol oxidase onto TiO2NPs, modified Pencil Graphite electrode and its application in construction of an amperometric biosensor for determination of serum cholesterol