The Biological and Clinical Significance of Glutaminase in Luminal Breast Cancer

Masisi, Brendah K.
El Ansari, Rokaya
Alfarsi, Lutfi
Craze, Madeleine L.
Jewa, Natasha
Oldfield, Andrew
Cheung, Hayley
Toss, Michael
Rakha, Emad A.
Green, Andrew R.

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Publication date

August 2021

DOI

10.3390/cancers13163963

Publisher

MDPI AG

Abstract

Glutamine metabolism has a key role in the regulation of uncontrolled tumour growth. This study aimed to evaluate the expression and prognostic significance of glutaminase in luminal breast cancer (BC). The glutaminase isoforms (GLS/GLS2) were assessed at genomic/transcriptomic levels, using METABRIC (n=1 398) and GeneMiner datasets (n=4 712), and protein using immunohistochemistry in well characterised cohorts of Oestrogen Receptor-positive/HER2-negative BC patients: ductal carcinoma in situ (DCIS; n=206) and invasive breast cancer (IBC; n=717). Glutaminase expression was associated with clinicopathological features, patient outcome and glutamine-metabolism related genes. In DCIS, GLS alone and GLS+/GLS2- expression was a risk factor for s...