Friable and embryogenic callus induction and plant regeneration from rubber tree clone Reyan88-13 Hevea brasiliensis

The inner integument from the young seeds of rubber tree clone Reyan88-13 was used to induce embryogenic callus. The different culture parameters such as the different types and concentrations of plant growth regulators and different calcium concentrations were studied in embryogenic callus induction media and subculture media. The proper culture parameters were determined. Friable and embryogenic callus were gradually obtained via continuous subculture and selection during 6 months. The suitable friable and embryogenic callus induction medium was: MH basal components, supplemented with 0.5 mg/L KT,2.0 mg/L 2,4-D, 11.0 mmol/L CaCl2, 80.0 g/L sucrose and 2.0 g/L Phytagel. Long-term subculture had been carried on for the friable and embryogenic calli. Histological sections proved that the calli proliferated by long -term subculture maintained the embryogenic characters. The friable and embryogenic calli of rubber tree clone Reyan88-13 that had been subcultured for more than 2 years were used for inducing somatic embryos. More than 16 000 somatic embryos were acquired, then 115 regeneration plantlets were obtained. (Résumé d'auteur