Fluorogenic probes for live-cell imaging of the cytoskeleton.
- Lukinavicius, G.
- Reymond, L.
- D'Este, E.
- Masharina, A.
- Göttfert, F.
- Ta, H.
- Guether, A.
- Fournier, M.
- Rizzo, S.
- Waldmann, H.
- Blaukopf, C.
- Sommer, C.
- Gerlich, D.
- Arndt, H.
- Hell, S.
- Johnsson, K.
Publication date
July 2014
DOIAbstract
We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells
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