Nat Biotech
- Deutsch, E.
- Ball, C.
- Berman, J.
- Bova, G.
- Brazma, A.
- Bumgarner, R.
- Campbell, D.
- Causton, H.
- Christiansen, J.
- Daian, F.
- Dauga, D.
- Davidson, D.
- Gimenez, G.
- Goo, Y.
- Grimmond, S.
- Henrich, T.
- Herrmann, B.
- Johnson, M.
- Korb, M.
- Mills, J.
- Oudes, A.
- Parkinson, H.
- Pascal, L.
- Pollet, N.
- Quackenbush, J.
- Ramialison, M.
- Ringwald, M.
- Salgado, D.
- Sansone, S.
- Sherlock, G.
- Stoeckert Jr, C.
- Swedlow, J.
- Taylor, R.
- Walashek, L.
- Warford, A.
- Wilkinson, D.
- Zhou, Y.
- Zon, L.
- Liu, A.
- True, L.
DOIAbstract
One purpose of the biomedical literature is to report results in sufficient detail that the methods of data collection and analysis can be independently replicated and verified. Here we present reporting guidelines for gene expression localization experiments: the minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE). MISFISHIE is modeled after the Minimum Information About a Microarray Experiment (MIAME) specification for microarray experiments. Both guidelines define what information should be reported without dictating a format for encoding that information. MISFISHIE describes six types of information to be provided for each experiment: experimental design, biomaterials and treatmen...
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