Cryptotomography: Reconstructing 3D Fourier Intensities from Randomly Oriented Single−Shot Diffraction Patterns
- Loh, N. D.
- Bogan, M. J.
- Elser, V.
- Barty, A.
- Boutet, S.
- Bajt, S.
- Hajdu, J.
- Ekeberg, T.
- Maia, F. R.
- Schulz, J.
- Seibert, M. M.
- Iwan, B.
- Timneanu, N.
- Marchesini, S.
- Schlichting, Ilme
- Shoeman, Robert L.
- Lomb, Lukas
- Frank, M.
- Liang, M.
- Chapman, H. N.
DOIAbstract
We reconstructed the 3D Fourier intensity distribution of monodisperse prolate nanoparticles using single−shot 2D coherent diffraction patterns collected at DESY's FLASH facility when a bright, coherent, ultrafast x−ray pulse intercepted individual particles of random, unmeasured orientations. This first experimental demonstration of cryptotomography extended the expansion−maximization−compression framework to accommodate unmeasured fluctuations in photon fluence and loss of data due to saturation or background scatter. This work is an important step towards realizing single−shot diffraction imaging of single biomolecules
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