Novel neutrophil-derived proteins in bronchoalveolar lavage fluid indicate an exaggerated inflammatory response in pediatric cystic fibrosis patients
- McMorran, Brendan J
- Ouvry Patat, Severine A
- Robertson, Colin F
- Hume, David A
- Borchers, Christoph H
- Wainwright, Claire E
- Wainwright, Brandon J
- Carlin, John B
- Grimwood, Keith
- Jones, Alun
- Armstrong, David S
- Galati, John C
- Cooper, Peter J
- Byrnes, Catherine A
- Francis, Paul W
DOIAbstract
Background: Airway inflammation in cystic fibrosis (CF) is exaggerated and characterized by neutrophil-mediated tissue destruction, but its genesis and mechanisms remain poorly understood. To further define the pulmonary inflammatory response, we conducted a proteome-based screen of bronchoalveolar lavage fluid (BALF) collected from young children with and without CF experiencing endobronchial infection. Methods: We collected BALF samples from 45 children younger than 5 years and grouped them according to the presence of respiratory pathogens: ≥1 × 10⁵ colony-forming units (CFU)/mL BALF (18 and 12 samples with and without CF, respectively) and <1 × 10⁵ CFU/mL (23 and 15 samples). BALF proteins were analyzed with SELDI-TOF mass spectrometry ...
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