Automated detection of rare-event pathogens through time-gated luminescence scanning microscopy
- Lu, Yiqing
- Jin, Dayong
- Leif, Robert C
- Deng, Wei
- Piper, James A
- Yuan, Jingli
- Duan, Yusheng
- Huo, Yujing
DOIAbstract
Many microorganisms have a very low threshold (100 μs, and the autofluorescence backgrounds, <0.1 μs, to render background autofluorescence invisible to the detector. Rather than having to resort to sophisticated imaging analysis, the background-free feature allows a single-element photomultiplier to locate rare-event cells, so that requirements for data storage and analysis are minimized to the level of image confirmation only at the final step. We have evaluated this concept in a prototype instrument using a 2D scanning stage and applied it to rare-event Giardia detection labeled by a europium complex. For a slide area of 225 mm², the time-gated scanning method easily reduced the original 40,000 adjacent elements (0.075 mm x 0.075 mm) dow...
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