In vitro propagation of Cordia verbenaceae L. (Boraginaceae).

Clonal propagation by tissue culture is useful to regenerate large populations of plants with similar characteristics. There are no previous reports of micropropagation of Cordia verbenacea. In this study, in vitro techniques were applied multiply this important Brazilian medicinal plant. Apical and nodal segments were cultured on Murashige and Skoog solid (0,6%) medium (MS) supplemented with 0.1, 1.0, 5.0 and 10.0 µM kinetin and 0.01µM naphthaleneacetic acid (NAA). Segments apical yielded more propagules than nodal segments. The number and length of propagules increased with in kinetin levels at 5 µM kinetin, decrease with more concentration. The treatment containing 5 µM kinetin and 0.01µM NAA yielded 2.7 propagules per explant. Propagules rooted on MS medium without growth regulators. Finally, 90-95% of the micropropagated plants survived when transferred to greenhouse conditions