Modelling the mortality of Hylotrupes bajulus (L.)larvae exposed to anoxic treatment for disinfestation of wooden art objects

Experiments were conducted to quantify the effect of several variables on the mortality of insects exposed to an anoxic treatment in order to generate a model linking mortality to these variables. This study aims to explore the possible interest of using such a model to determine the characteristics of treatment (especially duration) needed to guarantee insect mortality with a given level of probability. Trials were performed on Hylotrupes bajulus larvae, which is a widespread species known for its high tolerance to anoxic conditions. The studied variables are the initial mass of the larvae, the treatment temperature (21, 30 and 40 °C), the treatment duration (four durations for each temperature tested) and whether the larva is held in wood or in a petri dish (directly exposed to anoxic atmosphere) during the experiment. It was found that, while the last variable is not correlated with mortality, treatment duration and temperature are significantly and positively correlated with it. Larvae with higher body mass were also shown to have a better resistance to the treatment. Based on these results, a model including insect initial mass, treatment temperature and duration, together with the interaction between these two variables, was determined. This relatively simple model appeared to be a useful tool in overcoming the difficulty in defining the modalities for anoxic treatment in order to reach a given level of mortality