In vitro and in vivo antileishmanial activities of a Brazilian green propolis extract

PONTIN, Karina
SILVA FILHO, Ademar A. da
SANTOS, Fransergio Francisco
SILVA, Marcio Luis Andrade e
CUNHA, Wilson Roberto
NANAYAKKARA, N. P. Dhammika
BASTOS, Jairo Kenupp
ALBUQUERQUE, Sergio de

Publication date

January 2008

Publisher

Springer Fachmedien Wiesbaden GmbH

Abstract

The in vitro antileishmanial activity of Brazilian green propolis hydroalcoholic extract (BPE) were carried out on Leishmania (Viannia) braziliensis against both promastigote (doses ranging from 1 to 750 mu g mL(-1)) and amastigote (10, 100, and 250 mu g mL(-1)) assays in comparison with the positive (amphotericin B) and negative (dimethyl sulfoxide at 1% in physiologic solution) control groups. BPE displayed in vitro antileishmanial activities against promastigote forms of the parasite (p<0.05). However, it was inactive against its amastigote ones. In the in vitro cytotoxicity assay against Vero cells, BPE showed no cytotoxicity in the maximum doses tested. The high-performance liquid chromatography analysis allowed the identification of c...