(1)H NMR determination of beta-N-methylamino-L-alanine (L-BMAA) in environmental and biological samples

MOURA, Sidnei
ULTRAMARI, Mariah de Almeida
PAULA, Daniela Mendes Louzada de
YONAMINE, Mauricio
PINTO, Ernani

Publication date

January 2009

Publisher

PERGAMON-ELSEVIER SCIENCE LTD

Abstract

A nuclear magnetic resonance ((1)H NMR) method for the determination of beta-N-methylamino-L-alanine (L-BMAA) in environmental aqueous samples was developed and validated. L-BMAA is a neurotoxic modified amino acid that can be produced by cyanobacteria in aqueous environments. This toxin was extracted from samples by means of solid-phase extraction (SPE) and identified and quantified by (1)H NMR without further derivatization steps. The lower limit of quantification (LLOQ) was 5 mu g/mL Good inter and intra-assay precision was also observed (relative standard deviation <8.5%) with the use of 4-nitro-DL-phenylalanine as an internal standard (IS). This method of 1H NMR analysis is not time consuming and can be readily utilized to monitor L-BM...

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(1)H NMR determination of beta-N-methylamino-L-alanine (L-BMAA) in environmental and biological samples

Abstract

Extracted data

(1)H NMR determination of beta-N-methylamino-L-alanine (L-BMAA) in environmental and biological samples

Abstract

Extracted data

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