Influência do comprimento do material obturador remanescente no canal radicular e preparo do espaço protético na infiltração coronária do Enterococcus Faecalis

This study evaluated the sealing ability of different lengths of remaining root canal filling and post space preparation against coronal leakage of Enterococcus faecalis. Forty-one roots of maxillary incisors were biomechanically prepared, maintaining standardized canal diameter at the middle and coronal thirds. The roots were autoclaved and all subsequent steps were undertaken in a laminar flow chamber. The canals of 33 roots were obturated with AH Plus sealer and gutta-percha. The root canal fillings were reduced to 3 predetermined lengths (n=11): G1=6 mm, G2=4 mm and G3=2 mm. The remaining roots served as positive and negative controls. Bacterial leakage test apparatuses were fabricated with the roots attached to Eppendorf tubes keeping 2 mm of apex submerged in BHI in glass flasks. The specimens received an E. faecalis inoculum of 1 x 107 cfu/mL every 3 days and were observed for bacterial leakage daily during 60 days. Data were submitted to ANOVA, Tukey's test and Fisher's test. At 60 days, G1 (6 mm) and G2 (4 mm) presented statistically similar results (p>0.05) (54.4% of specimens with bacterial leakage) and both groups differed significantly (p<0.01) from G3 (2 mm), which presented 100% of specimens with E. faecalis leakage. It may be concluded that the shortest endodontic obturation remnant leaked considerably more than the other lengths, although none of the tested conditions avoids coronal leakage of E. faecalis.Este estudo avaliou a capacidade de diferentes de remanescentes de material obturador do canal radicular e preparo do espaço protético na infiltração do Enterococcus faecalis. Quarenta e uma raízes de incisivos superiores foram preparadas biomecanicamente, mantendo-se diâmetro padronizado nos terços médio e cervical. As raízes foram autoclavadas e todos os passos subseqüentes foram realizados em capela de fluxo laminar. Os canais de 33 raízes foram obturadors com AH Plus e guta-percha. As obturações foram reduzidas a 3 comprimentos (n=11): G1=6 mm, G2=4 mm e G3=2 mm. As raízes remanescentes serviram de controles positivo e negativo. O dispositivo para testar a microinfiltração bacteriana foi confeccionado com as raízes fixas a Eppendorfs, mantendo-se 2 mm do ápice submergido em vidro contendo BHI. Os dentes receberam o inóculo de 1 x 10(7) UFC/ml de E. faecalis a cada 3 dias, com observação diária por 60 dias. Os dados obtidos foram submetidos à análise de variância, teste de Tukey e Fisher. Foi possível observar que aos 60 dias, o G1 (6 mm) e G2 (4 mm) apresentou resultados estatisticamente semelhantes (p>0,05) (54% dos espécimes com infiltração bacteriana) e ambos os grupos foram diferentes estatisticamente (p