Polymerase chain reaction (PCR) is highly sensitive for diagnosis of mucosal leishmaniasis.
- Oliveira, Jene Greyce Souza de
- Novais, Fernanda Oliveira
- Oliveira, Camila Indiani de
- Cruz Junior, Antonio Clementino da
- Campos, Léon Fábio
- Rocha, Any V. da
- Boaventura, Viviane Sampaio
- Noronha, Almério Libório Lopes de
- Costa, Jackson Mauricio Lopes
- Barral, Aldina Maria Prado
Publication date
January 2005
DOI Publisher
Elsevier BV ISSN
0001-706X Citation count (estimate)
67Abstract
We evaluated the use of polymerase chain reaction (PCR) for diagnosis of mucosal leishmaniasis (ML) in an endemic area in Acre, Brazil, where Leishmania braziliensis is present. Leishmania DNA was detected 34 of 35 cases, yielding a positivity rate of 97.1%, which was higher than the positivity rates for all of the other diagnostic methods studied, namely Montenegro skin test (MST), anti-Leishmania serological testing and microscopic examination of lesion biopsy specimens. These findings have led us to propose guidelines for the diagnosis of ML that use PCR as the principal method of parasitological confirmation of cases
Add to ORKG