Contamination of expressed human milk by mycelial fungi

Objetivo: caracterizar os g??neros de fungos miceliais encontrados em amostras de leite humano ordenhado, recebidas a partir de coleta domiciliar, pelo Banco de Leite Humano do Instituto Fernandes Figueira. M??todos: foram estudadas 821 amostras de leite humano ordenhado, obtidas ao acaso, a partir de frascos coletados nos domic??lios pelas pr??prias doadoras. Foram realizadas pesquisas de bolores e leveduras e de microorganismos mes??filos. A partir das amostras de leite humano ordenhado, foram isoladas 48 cepas de fungos miceliais, que foram identificadas por t??cnicas-padr??o de laborat??rio. Resultados: as an??lises microbiol??gicas revelaram a ocorr??ncia de bolores e leveduras em 43 (5,2%) das amostras, com contagens atingindo a ordem de 103 UFC/ml, e foram identificados os microorganismos Aspergillus Grupo Niger (6,3%), Aspergillus sp. (4,2%), Paecilomyces sp. (12,6%), Penicillium sp. (60,4%), Rhizopus sp. (2,0%) e Syncephalastrum sp. (14,5%). Quatro amostras apresentavam mais de um tipo de fungo micelial. Conclus??o: a presen??a de bolores e leveduras no leite humano ordenhado nos domic??lios sugere que as condi????es higi??nicas do local de coleta podem contaminar o leite. Portanto, quando se trata do repasse do produto cru para beb??s prematuros hospitalizados, ?? fundamental observar as condi????es de coleta, estocagem e transporte para evitar a presen??a e as conseq????ncias da multiplica????o de contaminantes.Objective: to characterize the genera of mycelial fungi detected in expressed human milk received at the human milk bank of Instituto Fernandes Figueira after home collection. Methods: we studied 821 expressed human milk samples randomly obtained from flasks filled by the donors at home. The possible presence of molds, yeasts and mesophilic microorganisms was investigated. A total of 48 strains of mycelial fungi were isolated from the human milk bank samples and identified through standard laboratory techniques. Results: microbiological analysis revealed the occurrence of molds and yeasts in 43 samples (5.2%), with counts reaching 103 CFU/ml. The following microorganisms were identified: Aspergillus niger group (6.3%), Aspergillus sp. (4.2%), Paecilomyces sp. (12.6%), Penicillium sp. (60.4%), Rhizopus sp. (2.0%), and Syncephalastrum sp. (14.5%). Four samples showed the presence of more than one mycelial fungus type. Conclusions: the presence of molds and yeasts in human milk manually expressed at home suggests that the hygiene conditions of the collection site may contaminate milk. Thus, when hospitalized premature babies receive the raw product, it is very important to observe the collection, storage and transport conditions in order to avoid the presence and consequences of contaminants increase