Antifungal action of essential oils of Origanum vulgare and Rosmarinus officinalis against isolates of Pythium insidiosum and Dermatophytes

O presente estudo teve como objetivos determinar a composi????o qu??mica e avaliar a a????o antif??ngica dos ??leos essenciais de Origanum vulgare (or??gano) e Rosmarinus officinalis (alecrim). Os ??leos foram analisados por cromatografia gasosa e identificados por compara????o com o tempo de reten????o de padr??es. A avalia????o da atividade antif??ngica foi realisado o m??todo de microdilui????o em caldo, sendo utilizados nove isolados de dermat??fitos e nove isolados de Pythium insidiosum provenientes de casos cl??nicos em animais. Os ??leos foram submetidos a uma s??rie de dez dilui????es em logaritmo de base 2, no meio RPMI 1640, obtendo-se concentra????es de 30- 0,03??l/mL. As microplacas foram incubadas a 32??C por 72 h e a susceptibilidade foi expressa pela concentra????o inibit??ria m??nima (CIM). Os principais constituintes dos ??leos de R. oficinalis foram c??nfora (51,52%), verbenona (11,84%), 1,8-cineol (8,94%), mirceno (4,50%), ??-terpineol (4,39%) e borneol (2,68%) e para o O. vulgare foram 4-terpineol (27,67%), gama-terpineno (4,10%), timol (0,58%) e carvacrol (21,58%). Para os Dermat??fitos o CIM do R. Officinallis variou entre 30 a 3 ??l/ml e para o O. vulgare variou entre 3 a 0,3 ??l/mL. No caso do P. insidiosum o CIM do R. Officinallis variou entre 3 a 1,5 ??l/ml e para o O. vulgare variou entre 3 a 0,75 ??l/mL. De acordo com os resultados obtidos, pode-se afirmar que o ??leos testados apresentam atividade antif??ngica em dermat??fitos e P. insidiosum. Os resultados estimulam novos estudos, especialmente em rela????o a P. insidiosum, j?? que ainda n??o est?? dispon??vel nenhum tratamento eficaz.This study aimed to determine the chemical composition and evaluate the antifungal essential oils of Origanum vulgare (oregano) and Rosmarinus officinalis (rosemary). The oils were analyzed by gas chromatography and identified by comparison with the retention time of standards. To evaluate the antifungal activity method was used in broth, and used nine isolates of dermatophytes and nine isolates of Pythium insidiosum from clinical cases in animals. The oils were subjected to a series of ten dilutions in logarithm base 2, in RPMI 1640, resulting in concentrations from 30 to 0, 03 ??lmL-1. The microplates were incubated at 32??C for 72 h and susceptibility was expressed by the minimum inhibitory concentration (MIC). The main oil constituents R. officinalis were camphor (51.52%), verbenone (11.84%), 1,8-cineole (8.94%), myrcene (4.50%), ??-terpineol (4.39%) and borneol (2.68%) and O. vulgare were 4-terpineol (27.67%), gamma-terpinene (4.10%), thymol (0.58%) and carvacrol (21.58%). For dermatophytes the MIC R. Officinallis ranged from 30 to 3 ??/lmL and O. vulgare varied from 3 to 0.3 ??L/ml. In the case of P. insidiosum MIC R. Officinallis ranged from 3 to 1.5 ??l/mL and O. vulgare varied from 3 to 0.75 ??l/mL. According to the results obtained, it can be argued that the tested oils have antifungal activity on dermatophytes and P. insidiosum. The results should stimulate further research, especially in relation to P. insidiosum, as yet no effective treatment is available