Anaemia focusing on macrocytic anaemia

The content of my thesis are macrocytic anaemia. I deal with overall issues of anaemia because it is a widespread disease. According to MCV, I divided individual anaemia into microcytic, normocytic and macrocytic. Then I focused only on the topic of macrocytic anaemia. Macrocytic anaemia is not a frequently occurring type of anaemia. The main causes of macrocytosis include deficiency of folic acid and vitamin B12. I worked with a group of patients, who had examined blood counts for one month in the hospital České Budějovice a. s. In these patients, I examined the frequency of anaemia, which I further divided by MCV into microcytic, normocytic and macrocytic. Then I focused on the macrocytic anaemias. I worked with an automated hematology analyzer Unicel DxH 800 Beckman Coulter. In 12 % of all patients was detected macrocytic anaemia. According my observing, this disease appears more often with men and mostly in the age of 50 and older. In all cases I observed severity of this illness. Majority of patients was examined either for mild degree, or for moderate degree of anaemia. Severe cases were only rare. After that I traced back, if causes of macrocytosis were examined in cases, when patient suffer from anaemia. I worked with ADVIA analyzer and I found out that only 12 % patients were examined for vitamin B12 deficiency and only 11 % were examined for folic acid deficiency. I was wondering, why these parameters weren't examined. I watched therefore, which department examine these two parameters and if there is a connection between frequency of requests for this examination, together with the value of MCV or severity of anaemia. Based on my results, I realized that in cases of macrocytic anaemia often no search was done and that reduces the effectiveness of treatment