Production of extracellular hydrolases by specific mould species

This bachelor's thesis is focused on studying the possibilities of producing extracellular hydrolytic enzymes by the fungus Penicillium lilacinum on two substrates. The theoretical part is focused on the characterization of selected hydrolytic enzymes, their characteristics, the possibilities of production and their applications. In the experimental part the production of hydrolytic enzymes by the fungus Penicillium lilacinum was performed in mineral medium and in a medium wherein as substrate yeast strains Sporobolomyces roseus and Rhodotorula glutinis were used. During cultivation process, production of cellulases, amylases, xylanases, lipases, proteases, and mannases was monitored. Samples were taken on 3rd, 5th, 8th, 10th and 12th day of cultivation. Production varies depending on time and substrate type. Amylase, cellulose and xylanase activity was measured in two steps - at the beginning of culture (3 to 5 day) and at the end of cultivation. The rest of enzymes were detected mainly in the 8th to 10th day of culture. The best three samples with the highest value of enzyme activities were further used for lyofilization and purification by ultrafiltration. Further, enzymes were tested for disruption of yeast cell wall. In the conclusion, the yeast protoplasts of yeast strains Rhodotorula glutinis a Sporobolomyces roseus were prepared successfully