Determination of musk compounds in biotic matrices

This diploma thesis deals with actual issues of the contamination in the environment with synthetic fragrances. Musk compounds are infiltrated to many environmental components (especially an aquatic ecosystem) because of their biological persistence and the ability of accumulation. This diploma thesis is focused on the selection and the optimization of method for the determination of musk compounds in real biotic matrices (fish). The isolation of analytes was realized by PSE method and the purification of extract was realized by the method of the adsorption column chromatography. Identification and quantification of individual musk compounds was carried out by GC-MS. In conclusion were results and the contamination of fish from the river Svratka disscissed and evaluated. These fish were caught at the inflow and at the outflow of the wastewater treatment plant