Encapsulation of some enzymes into organic particles

This bachelor thesis is focused on encapsulation of some enzymes into organic particles. The theoretical part is aimed to enzymes, their general characteristics, structure, activity and use, and to encapsulation techniques. Some methods used for analysis of particles, namely the determination of the distribution of particle size using dynamic light scattering, and determining the stability of the particles by the zeta potential, are described too. The experimental part describes methods of preparing particles, methods of determining the encapsulation efficiency and methods for determining particle size and stability. During the experiment part four types of enzymes were encapsulated, partly by manual preparation and partly by encapsulator. Encapsulation efficiency, size and stability of prepared particles were determined too. The particles were exposed to artificial intestinal, gastric and bile juices, as well as the effect of model foods. Subsequently, their long-term stability was observed