Verification of method of reconstruction of spatial distribution of refractive index by a holographic microscope

Abstract The subject of the bachelor thesis is an application of transmission digital holographic microscope for verification of reconstruction of spatial distribution of the refractive index. Using eccentric pinhole as a light source is the object illuminated by a plane wave inclined to the optical axis. Different rotations of a pinhole gives us projections of the object. We have tried to reconstruct the refractive index using tomographic methods. This thesis contains design of a rotating pinhole aperture. The illumination and mounting was solved and a functionality was also proved. Experimental part deals with the reconstruction of refractive index of a tested object